Effect of phytohaemagglutinin and tuberculin on macromolecule synthesis in human lymphocyte cultures.

Effect of E. coli antigens, tuberculin, and phytohaemagglutinin upon ulcerative colitis lymphocytes.

The activation and reactivation of peripheral lymphocytes in culture.

Human lymphocytes stimulated for 16 h and then cultured without stimulant showed maximal activity on days 2-3 following a stimulus of phytohaemagglutinin (PHA) and on days 3-4 following a stimulus of staphylococcal nitrate (SF). At low dosage of stimulant the response of the cells was less marked but persisted for a longer period than at high dosage. The pattern of response is discussed in rela...

Phytohaemagglutinin and transfer factor in the leucocyte migration inhibition test in patients with sarcoidosis.

A new system of phytohaemagglutinin stimulation in vitro using the leucocyte migration inhibition test has been applied to patients with active and inactive sarcoidosis and subjects with a persistently negative response to tuberculin. Patients with active sarcoidosis and the tuberculin-negative subjects have a depressed response to the mitogen. This response is improved by the addition of trans...

Effect of amphotericin B and clotrimazole on lymphocyte stimulation.

Human lymphocytes were stimulated in vitro by phytohemagglutinin, concanavalin A, pokeweed mitogen, purified protein derivative-tuberculin, and allogenic cells. The deoxyribonucleic acid synthesis of the lymphocytes was inhibited in increasing degree by 4 to 8 mug of amphotericin B per ml of culture irrespective of lymphocyte stimulant used. The effect of clotrimazole on the deoxyribonucleic ac...

Effect of cycloheximide on protein and ribonucleic acid synthesis in cultured human lymphocytes.

1. Phytohaemagglutinin stimulates the transformation into blast cells of human lymphocytes incubated in vitro. This transformation is accompanied by an increase in the incorporation of [(14)C]leucine into protein and [(3)H]uridine into RNA. 2. The incorporation of [(14)C]leucine by cultures grown in the presence or absence of phytohaemagglutinin is inhibited to the same extent by cycloheximide,...